產(chǎn)品詳情
簡單介紹：
重組鼠白細(xì)胞介素-13蛋白與其它公司提供的重組蛋白不同，rMuIL-13蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨(dú)有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠白細(xì)胞介素-13蛋白（全長序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測、ELISA等試驗(yàn)中.
詳情介紹：
重組鼠白細(xì)胞介素-13蛋白
| Species | Murine |
| Accession | P20109 |
| GeneID | 16163 |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 12.3 kDa, a single non-glycosylated polypeptide chain containing 111 amino acids. |
| Quantity | 重組鼠白細(xì)胞介素-13蛋白2μg/10μg/1000μg |
| AA Sequence | MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/ml, corresponding to a specific activity of > 2.5 × 105 IU/mg. |
| Physical Appearance | 重組鼠白細(xì)胞介素-13蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rMuIL-13 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重組鼠白細(xì)胞介素-13蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠白細(xì)胞介素-13蛋白 | |
| SDS-PAGE |
|
| Reference |
1. Schmutz J, Martin J, Terry A, et al. 2004. Nature, 431: 268-74. 2. Wynn TA. 2003. Annu Rev Immunol, 21: 425-56. 3. Lakkis FG, Cruet EN, Nassar GM, et al. 1997. Biochem Biophys Res Commun, 235: 529-32. |
| Background | Murine Interleukin-13 (IL-13) is expressed by the IL13 gene located on the chromosome 11 and secreted by many cell types, especially T helper type 2 (Th2) cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 has been implicated as a key factor in asthma, allergy, atopy and inflammatory response, establishing the protein as a valuable therapeutic target. Human, mouse and rat IL-3 share low homology, but have cross species activity. |
